Volume 32 Issue 1 - January 3, 2020 PDF
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Generation of 3D Hepatic Multicellular Tumor Spheroids for Discovery of Novel Therapeutics
Chiao-Yi Chiu1, Ying-Chi Chen1, Kuang-Wei Wu1, Wen-Chien Hsu1, Hong-Ping Lin2, Hsien-Chang Chang1,3,4,Yung-Chun Lee4,5, Yang-Kao Wang6, Ting-Yuan Tu1,3,4,*
1Department of Biomedical Engineering, National Cheng Kung University
2 Department of Chemistry, National Cheng Kung University
3 Medical Device Innovation Center, National Cheng Kung University
4 Center for Micro/Nano Technology Research, National Cheng Kung University
5 Department of Mechanical Engineering, National Cheng Kung University
6 Department of Cell Biology and Anatomy, College of Medicine, National Cheng Kung University
* Author to whom correspondence should be addressed
 
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Three-dimensional cell culture has revolutionized biomedical research for more than a decade. In the past half century, scientists have mostly cultured cells in two-dimensional plastic dishes for research. However, this culture method is very different from the conditions for the cells to grow inside the human body. Therefore, in order to better simulate the physiological micro-environment of in-vivo diseases, the three-dimensional culture method has gradually emerged. Taking cancer research as an example, a three-dimensional clustering of cells (multicellular tumor spheroids) can recapitulate several key tumor characteristics, such as the transfer of nutrients and oxygen. This study presented a simple and economical microstructure preparation method to produce hepatic tumor spheroids of uniform size, which can be easily incorporated into a conventional laboratory setting and used to develop novel therapeutics for liver cancer. In this study, Huh-7 hepatocellular carcinoma cells could grow into tumor spheroids of 250-520 m within five days of culture. With the combination of concanavalin A and silica carbo hallow spheres, the tumor spheroids could be treated by the photo-thermal therapy.

Schematic illustrations and an image of the rapid laser ablation of microwells integrated with standard culture plasticware for the identification of novel therapeutics through high-throughput screening drug screening and photothermal treatment.
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